Development and validation of genic-SSR markers in sesame by RNA-seq
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چکیده
منابع مشابه
Development of Polymorphic Genic SSR Markers by Transcriptome Sequencing in the Welsh Onion (Allium fistulosum L.)
Transcriptome analysis is an efficient way to explore molecular markers in plant species, for which genome sequences have not been published. To address the limited number of markers published for the Welsh onion, this study found 6486 loci of genic simple sequence repeats (SSR), which consisted of 1–5 bp repeat motifs, based on next-generation sequencing (NGS) technology and the RNA-Seq approa...
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UNLABELLED PREMISE OF THE STUDY Genic microsatellites or simple sequence repeat (genic-SSR) markers were developed in boxwood (Buxus taxa) for genetic diversity analysis, identification of taxa, and to facilitate breeding. • METHODS AND RESULTS cDNA libraries were developed from mRNA extracted from leaves of Buxus sempervirens 'Vardar Valley' and sequenced using the Illumina MiSeq system. ...
متن کاملDevelopment and characterization of polymorphic genic-SSR markers in Larix kaempferi.
New simple sequence repeat (SSR) markers were developed in the Japanese larch (Larix kaempferi) using unigene sequences for further genetic diversity studies and the genetic improvement of breeding programs. One thousand two handred and thirty five (1235) primer pairs were tested and 165 successfully identified in L. kaempferi. Out of the amplified candidate markers, 145 (90.6%) exhibited polym...
متن کاملDevelopment and validation of SSR markers for Coffea arabica L
With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with a...
متن کاملDevelopment of Genic and Genomic SSR Markers of Robusta Coffee (Coffea canephora Pierre Ex A. Froehner)
Coffee breeding and improvement efforts can be greatly facilitated by availability of a large repository of simple sequence repeats (SSRs) based microsatellite markers, which provides efficiency and high-resolution in genetic analyses. This study was aimed to improve SSR availability in coffee by developing new genic-/genomic-SSR markers using in-silico bioinformatics and streptavidin-biotin ba...
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ژورنال
عنوان ژورنال: BMC Genomics
سال: 2012
ISSN: 1471-2164
DOI: 10.1186/1471-2164-13-316